Xirmada Quantitative ee Canine Cortisol (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cCor)

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Canine Cortisol hal tallaabo tijaabo

 

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10 imtixaan/sanduuqa


Faahfaahinta Alaabta

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Ujeedada Imtixaanka

Canine Cortisol (cCortisol) waa hoormoon steroid ah oo ay soo saarto kortex adrenal canine.Cortisol waxay caawisaa joogteynta heerka sonkorta dhiigga ee joogtada ah, waxay yaraynaysaa bararka waxayna ka caawisaa jidhka inuu maareeyo walbahaarka.Iyadoo xaalado kala duwan oo ay sababaan heerarka aan caadiga ahayn ee hoormoonka khamriga lagu magacaabo Cushing syndrome (CS), eeyaha iyo bisadaha labaduba waxay ku dhici karaan CS, taas oo ku badan eeyaha marka loo eego bisadaha.Eeyaha dhexe iyo da'da (qiyaastii 7 ilaa 12 sano jir)
Waxay u badan tahay inuu ku dhaco cudurka.Cudurku si tartiib tartiib ah ayuu u koraa, calaamadaha horena ma fududa in la ogaado.CS waxaa lagu baari karaa kiliinikada adrenocorticotropic hormone (ACTH) baaritaanka kicinta iyo dexamethasone tijaabinta, iyo noocyadeeda kala duwan: adrenal-dependent (ATH) iyo pituitary-dependent (PDH).

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Mabda'a Ogaanshaha

Alaabtani waxay isticmaashaa fluorescence immunochromatography si ay qiyaas ahaan u ogaato waxa ku jira cCortisol ee serum/plasma eeyga.Mabda'a aasaasiga ah: T iyo C waxay ku calaamadsan yihiin xuubka nitrocellulose, khadka T waxaa lagu dahaadhay cCortisol antigen a, suufka xiraha waxaa lagu buufiyaa nanomaterial fluorescent ah oo ku calaamadsan antibody b kaas oo si gaar ah u aqoonsan kara cCortisol.
cCortisol ee muunada marka hore waxa lagu calaamadeeyay nanomaterial.Ka-hortagga unugga b wuxuu isku xiraa inuu sameeyo kakan, ka dibna chromatography kor.Qalabku wuxuu la tartamayaa T-line antigen a lamana qabsan karo;Taa lidkeeda, marka aanu jirin cCortisol muunada, antibody b waxa ay xidhaa antigen a.Marka iftiinka kicinta la shido, maaddada nano waxay soo saartaa calaamada fluorescent, iyo xoogga calaamaduhu waxay u dhigantaa qiyaasta cCortisol ee muunada.


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