Qalabka Tiro-koobka Progesterone ee Canine (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cProg)

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Magaca: cProg hal tallaabo imtixaan

 

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Ujeedada ogaanshaha

Isku-duubnaanta progesterone-ka canine ee serum waxay la xiriirtaa heerka estrus-ka.Marka la barbardhigo LH, xoojinta cProg had iyo jeer waa kor u kaca inta lagu jiro estrus eyga dheddigga ah, taas oo sahlan in la raad raaco oo la beddeli karo wakhtiga dhabta ah Waqtiga ugu wanaagsan ee taranka waa 3-6 maalmood ka dib ugu sarreeya LH, taas oo ku xidhan dheddigga. xaaladda estrus ee eyga.Inta u dhaxaysa dheddigga kala duwan, Heerka progesterone ee u dhiganta wakhtiga ugu habboon ee taranka aad buu u kala duwanaa, guud ahaan wuxuu u dhexeeyaa 0-50ng / ml, laakiin sidoo kale waxaa jiray in ka badan intan oo kale, sidaas darteed, heerka keratinization ee epithelium siilka ayaa lagu daray la socoshada firfircoonida waqtiga dhabta ah ee uruurinta serum progesterone Habka qiimayntu waxa uu si weyn u wanaajin karaa ixtimaalka rimitaanka eeyga dheddigga ah.

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Mabda'a Ogaanshaha

Waxa ku jira cProg ee serum/plasma eeyga waxa tiro ahaan lagu ogaaday fluorescence immunochromatography.Mabda'a aasaasiga ah: Fiber Nitrate Waxaa jira khadadka T iyo C ee filimka cabbirka siday u kala horreeyaan, khadka Tna waxaa lagu dahaadhay cProg antigen a, kaas oo si gaar ah u aqoonsan kara cProg adoo ku buufinaya suufka.
cProg ee muunada marka hore waxaa lagu xidhay nanomaterial calaamadan antibody b si loo sameeyo Complex, ka dibna wajiga sare, kakan ayaa la tartamaya T-line antigen oo aan la qabsan karin;Taa baddalkeeda, marka aanu jirin muunad Marka uu jiro cProg, antibody b wuxuu ku xidhaa antigen a.Marka shucaaca iftiinka iftiinka, nanomaterial-ku wuxuu sii daayaa calaamada fluorescence.Awoodda calaamaduhu waxay si rogan u dhigantaa fiirsashada cProg ee muunadda.

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Natiijada Imtixaanka

Maadaama heerarka progesterone-ka ugu fiican ay la xiriiraan taranka, da'da iyo cabbirka eeyga, ma jiro qiimo go'an oo dhammaystiran, noocyada soo socda
Tixraaca kaliya, waxaa lagula talinayaa in shaybaar ama isbitaal kasta uu sameeyo cabbir tixraaceed oo u gaar ah sida waafaqsan rugta caafimaadka
Kulayl kuma jiro:<1ng/ml;
Estrus:xoojinta progesterone si tartiib tartiib ah ayuu u korayaa, wareegga guud ahaan waa 7-8 maalmood;Si loo hagaajiyo heerka guusha ee uurka, baaritaanka ugu horreeya ee progesterone
Xoojinta waa in ay ahaataa inta u dhaxaysa 10-50ng/ml, waxaana lagu talinayaa in la tarmo laba jeer.
10-30ng/ml:lammaane ugu horreeya gudaha 3h, lammaane labaad gudahood 48h;
30-60ng/ml:lammaane ugu horreeya gudaha 2h iyo lammaane labaad gudahood 24h;
60-80ng/ml:2h oo loogu talagalay lammaane.
Qiyaasta lagu ogaanayo xirmadan waa 1-80ng/ml.Haddii ay ka badato qiyaasta, ku dar <1ng/ml, ama> 80 ng/ml.


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