Feline N-terminal Pro-maskaxda Natriuretic Peptide Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (fNT-proBNP)

[Magaca Alaabta]

Magaca: feline NT-proBNP hal tallaabo imtixaan

 

[Qalabadaha Baakadaha]

10 imtixaan/sanduuqa


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Guud ahaan

Inta lagu jiro qarsoodiga, borotiinku wuxuu ku dheggan yahay BNP jir ahaan firfircoon (77th ilaa 108th amino acids) iyo n-terminal fragment NT-proBNP (1st ilaa 76th amino acids).Marka BNP, oo dhererkeedu yahay 32 amino acids, lagu sii daayo dhiigga, waxay ku xidhaa soo-qaadayaasheeda (NPRA iyo NPRB) waxayna nidaamisaa cadaadiska dhiigga iyada oo loo marayo habab kala duwan.NT-proBNP, oo dhererkeedu yahay 76 amino acids, ma laha dhaqdhaqaaq bayooloji, laakiin sababtoo ah waxay leedahay nus-nolosho ka dheer BNP, waxay ku habboon tahay calaamad muujinaysa cudurrada wadnaha ee kala duwan.Baaritaanka caafimaad ee xayawaanka rabaayada ah, dhiiga NT-proBNP ee eeyaha ayaa ka sarreeya 900 pmol/L, bisadahana waxay ka sarreeyaan 270 pmol/L, waxaana jira khatar sare oo ah cudurrada wadnaha la xiriira.Si kastaba ha noqotee, waa in la ogaadaa in sababta oo ah NT-proBNP ay ka soo baxdo kelyaha, marka xayawaanku ay ka cabanayaan cudurrada kelyaha la xiriira, xoojinta NT-proBNP ee jirka ayaa sidoo kale kordhin doonta oo been-abuurka ayaa ka muuqan doona baaritaanka.

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Mabda'a Ogaanshaha

Alaabtani waxay qabataa fluorescence immunochromatography si loo ogaado waxa ku jira fNT-proBNP ee serum/balaasmaha tiro ahaan.Mabda'a aasaasiga ah: Waxaa jira khadadka T iyo C ee xuubka fiber-ka nitric acid siday u kala horreeyaan, iyo khadka T waxaa lagu dahaadhay antibody kaas oo si gaar ah u aqoonsan fNT-proBNP.suufka isku dhafka ah waxaa lagu buufiyaa nanomaterial kale oo fluorescent nanomaterial ah oo calaamadeysan antibody b kaas oo si gaar ah u aqoonsan kara FDT-probNP.Muunadda, FDT-probNP marka hore waxay ku darsataa nanomaterial-ka calaamadsan antibody b si ay u sameeyaan kakan, ka dibna chromatography sare, kakanaanta waxay ku daraysaa T-line antibody A si ay u sameeyaan qaab-dhismeedka sandwich.Marka xamaasadda shucaaca iftiinka, nanomaterial-ka ayaa sii daaya calaamadaha fluorescence.Awoodda calaamaduhu waxay si togan ula xidhiidhaan fNT-proBNP ee muunada.


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